Effect of Ultrasonic Vibration on Proliferation of Cultured Cell

The effect of mechanical stimulation of vibration on proliferation of cells has been studied in vitro. To apply the vibration on the cells, a piezoelectric element was attached on the outside surface of the bottom of the culture plate of six wells. The piezoelectric element was vibrated by sinusoidal alternating voltage at 1.0 MHz generated by a function generator. Three kinds of cells were used in the experiment: C2C12 (mouse myoblast cell), L929 (fibroblast connective tissue of mouse), Hepa1-6 (mouse hepatoma cell). After incubation for 24 hours, cells were exposed to the ultrasonic vibration intermittently for three days: for thirty minutes per day. At the end of the experiment, the number of cells was counted by colorimetric method with the microplate photometer. The experimental results show that proliferation of cells is enhanced with mild vibration, and that the optimum vibration depends on the kind of cells.